Long-range distance measurements on nucleic acids in cells by pulsed EPR spectroscopy

Mapping the structure of nucleic acids: Pulsed electron–electron double-resonance (PELDOR) spectroscopy has been applied for the first time to map the global structure of nucleic acids inside Xenopus laevis oocytes. The distances measured in vitro and in cells are the same, which implies the existence of stable overall conformations of the hairpin RNA and the neomycin-sensing riboswitch studied.

  • Krstić I, Hänsel R, Romainczyk O, Engels JW, Dötsch V, Prisner TF. Long-range distance measurements on nucleic acids in cells by pulsed EPR spectroscopy. Angew Chem Int Ed Engl.; 50:5070 (2011).