- Solution State NMR
- Solid State NMR
- EPR
- DNP
- Structural Biology
- Chemical Biology
- Membrane Proteins
- RNA and DNA
- Molecular Biophysics
- Structure Calculation
- Cell-free expression

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins
Laguerre A, Löhr F, Henrich E, Hoffmann B, Abdul-Manan N, Connolly PJ, Perozo E, Moore JM, Bernhard F, Dötsch V., Structure. 2016 Oct 4;24(10):1830-1841.

Influence of Arrestin on the Photodecay of Bovine Rhodopsin
Chatterjee D, Eckert CE, Slavov C, Saxena K, Fürtig B, Sanders CR, Gurevich VV, Wachtveitl J, Schwalbe H., Angew Chem Int Ed Engl. 2015 Nov 9;54(46):13555-60.

In-cell NMR and EPR spectroscopy of biomacromolecules
Hänsel R, Luh LM, Corbeski I, Trantirek L, Dötsch V., Angew Chem Int Ed Engl. 2014 Sep 22;53(39):10300-14.

Coupled ATPase-adenylate kinase activity in ABC transporters
Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C, Nat Commun. 2016 Dec 22;7:13864.

Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy
Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C, Proc Natl Acad Sci U S A. 2015, 112, 9896-901.

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR
Maciejko J, Mehler M, Kaur J, Lieblein T, Morgner N, Ouari O, Tordo P, Becker-Baldus J, Glaubitz C., J Am Chem Soc. 2015, 137, 9032-43.

The Structural Basis of the Green-Blue Color Switching in Proteorhodopsin determined by NMR Spectroscopy
Mao, Jiafei; Do, Nhu-Nguyen; Scholz, Frank; Reggie, Lenica; Mehler, Michaela; Lakatos, Andrea; Ong, Yean-Sin; Ullrich, Sandra; Brown, Lynda; Brown, Richard; Becker-Baldus, Johanna; Wachtveitl, Josef; Glaubitz, Clemens, J Am Chem Soc. 2014, 136, 17578-90.

Double quantum coherence ESR spectroscopy and quantum chemical calculations on a BDPA biradical
Haeri HH, Spindler P, Plackmeyer J, Prisner T., Phys Chem Chem Phys. 2016 Oct 26;18(42):29164-29169.

Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
Joseph B, Tormyshev VM, Rogozhnikova OY, Akhmetzyanov D, Bagryanskaya EG, Prisner TF., Angew Chem Int Ed Engl. 2016 Sep 12;55(38):11538-42.

Carr-Purcell Pulsed Electron Double Resonance with Shaped Inversion Pulses
Spindler PE, Waclawska I, Endeward B, Plackmeyer J, Ziegler C, Prisner TF., J Phys Chem Lett. 2015 Nov 5;6(21):4331-5.

Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy
Joseph B, Sikora A, Bordignon E, Jeschke G, Cafiso DS, Prisner TF., Angew Chem Int Ed Engl. 2015 May 18;54(21):6196-9.

In-cell NMR and EPR spectroscopy of biomacromolecules
Hänsel R, Luh LM, Corbeski I, Trantirek L, Dötsch V., Angew Chem Int Ed Engl. 2014 Sep 22;53(39):10300-14.

Broadband inversion PELDOR spectroscopy with partially adiabatic shaped pulses
Spindler PE, Glaser SJ, Skinner TE, Prisner TF., Angew Chem Int Ed Engl. 2013 Mar 18;52(12):3425-9.

Bis-Gadolinium Complexes for Solid Effect and Cross Effect Dynamic Nuclear Polarization
Kaushik M, Qi M, Godt A, Corzilius B, Angew Chem Int Ed Engl. 2017 Apr 3;56(15):4295-4299.

Heteronuclear Cross-Relaxation under Solid-State Dynamic Nuclear Polarization
Daube D, Aladin V, Heiliger J, Wittmann JJ, Barthelmes D, Bengs C, Schwalbe H, Corzilius B., J Am Chem Soc. 2016 Dec 28;138(51):16572-16575.

Gd(iii) and Mn(ii) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins
Kaushik M, Bahrenberg T, Can TV, Caporini MA, Silvers R, Heiliger J, Smith AA, Schwalbe H, Griffin RG, Corzilius B., Phys Chem Chem Phys. 2016 Oct 21;18(39):27205-27218.

Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids
Corzilius B., Phys Chem Chem Phys. 2016 Oct 21;18(39):27190-27204.

Dynamic nuclear polarization of nucleic acid with endogenously bound manganese
Wenk P, Kaushik M, Richter D, Vogel M, Suess B, Corzilius B., J Biomol NMR. 2015 Sep;63(1):97-109.

Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy
Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C, Proc Natl Acad Sci U S A. 2015, 112, 9896-901.

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR
Maciejko J, Mehler M, Kaur J, Lieblein T, Morgner N, Ouari O, Tordo P, Becker-Baldus J, Glaubitz C., J Am Chem Soc. 2015, 137, 9032-43.

The Structural Basis of the Green-Blue Color Switching in Proteorhodopsin determined by NMR Spectroscopy
Mao, Jiafei; Do, Nhu-Nguyen; Scholz, Frank; Reggie, Lenica; Mehler, Michaela; Lakatos, Andrea; Ong, Yean-Sin; Ullrich, Sandra; Brown, Lynda; Brown, Richard; Becker-Baldus, Johanna; Wachtveitl, Josef; Glaubitz, Clemens, J Am Chem Soc. 2014, 136, 17578-90.

Dynamic nuclear polarization-enhanced NMR on aligned lipid bilayers at ambient temperature
Jakdetchai O, Denysenkov V, Becker-Baldus J, Dutagaci B, Prisner TF, Glaubitz C, J Am Chem Soc. 2014, 136(44):15533-6.

Dynamic nuclear polarization of (1)H, (13)C, and (59)Co in a tris(ethylenediamine)cobalt(III) crystalline lattice doped with Cr(III)
Corzilius B, Michaelis VK, Penzel SA, Ravera E, Smith AA, Luchinat C, Griffin RG., J Am Chem Soc. 2014 Aug 20;136(33):11716-27.
Mechanism of TAp73 inhibition by ΔNp63 and structural basis of p63/p73 hetero-tetramerization
Gebel J, Luh LM, Coutandin D, Osterburg C, Löhr F, Schäfer B, Frombach AS, Sumyk M, Buchner L, Krojer T, Salah E, Mathea S, Güntert P, Knapp S, Dötsch V., Cell Death Differ. 2016 Dec;23(12):1930-1940.

Design of photocaged puromycin for nascent polypeptide release and spatiotemporal monitoring of translation
Buhr F, Kohl-Landgraf J, tom Dieck S, Hanus C, Chatterjee D, Hegelein A, Schuman EM, Wachtveitl J, Schwalbe H., Angew Chem Int Ed Engl. 2015 Mar 16;54(12):3717-21.

Coupled ATPase-adenylate kinase activity in ABC transporters
Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C, Nat Commun. 2016 Dec 22;7:13864.

Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR
Joseph B, Tormyshev VM, Rogozhnikova OY, Akhmetzyanov D, Bagryanskaya EG, Prisner TF., Angew Chem Int Ed Engl. 2016 Sep 12;55(38):11538-42.

Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy
Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C, Proc Natl Acad Sci U S A. 2015, 112, 9896-901.

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR
Maciejko J, Mehler M, Kaur J, Lieblein T, Morgner N, Ouari O, Tordo P, Becker-Baldus J, Glaubitz C., J Am Chem Soc. 2015, 137, 9032-43.

Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy
Joseph B, Sikora A, Bordignon E, Jeschke G, Cafiso DS, Prisner TF., Angew Chem Int Ed Engl. 2015 May 18;54(21):6196-9.

The Structural Basis of the Green-Blue Color Switching in Proteorhodopsin determined by NMR Spectroscopy
Mao, Jiafei; Do, Nhu-Nguyen; Scholz, Frank; Reggie, Lenica; Mehler, Michaela; Lakatos, Andrea; Ong, Yean-Sin; Ullrich, Sandra; Brown, Lynda; Brown, Richard; Becker-Baldus, Johanna; Wachtveitl, Josef; Glaubitz, Clemens, J Am Chem Soc. 2014, 136, 17578-90.

A Stably Protonated Adenine Nucleotide with a Highly Shifted pKa Value Stabilizes the Tertiary Structure of a GTP-Binding RNA Aptamer
Wolter AC, Weickhmann AK, Nasiri AH, Hantke K, Ohlenschläger O, Wunderlich CH, Kreutz C, Duchardt-Ferner E, Wöhnert J., Angew Chem Int Ed Engl. 2017 Jan 2;56(1):401-404.

An intermolecular G-quadruplex as the basis for GTP recognition in the class V-GTP aptamer
Nasiri AH, Wurm JP, Immer C, Weickhmann AK, Wöhnert J., RNA. 2016 Nov;22(11):1750-1759.

Understanding How DNA Enzymes Work
Wirmer-Bartoschek J, Schwalbe H., Angew Chem Int Ed Engl. 2016 Apr 25;55(18):5376-7.

What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch
Duchardt-Ferner E, Gottstein-Schmidtke SR, Weigand JE, Ohlenschläger O, Wurm JP, Hammann C, Suess B, Wöhnert J., Angew Chem Int Ed Engl. 2016 Jan 22;55(4):1527-30.

Involvement of Long-Lived Intermediate States in the Complex Folding Pathway of the Human Telomeric G-Quadruple
Bessi I, Jonker HR, Richter C, Schwalbe H., Angew Chem Int Ed Engl. 2015 Jul 13;54(29):8444-8.

Building a stable RNA U-turn with a protonated cytidine
Gottstein-Schmidtke SR, Duchardt-Ferner E, Groher F, Weigand JE, Gottstein D, Suess B, Wöhnert J., RNA. 2014 Aug;20(8):1163-72.

Essential ribosome assembly factor Fap7 regulates a hierarchy of RNA-protein interactions during small ribosomal subunit biogenesis
Hellmich UA, Weis BL, Lioutikov A, Wurm JP, Kaiser M, Christ NA, Hantke K, Kötter P, Entian KD, Schleiff E, Wöhnert J., Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15253-8.

Three-state mechanism couples ligand and temperature sensing in riboswitches
Reining A, Nozinovic S, Schlepckow K, Buhr F, Fürtig B, Schwalbe H., Nature. 2013 Jul 18;499(7458):355-9.
Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family
Kehrloesser S, Osterburg C, Tuppi M, Schäfer B, Vousden KH, Dötsch V., Cell Death Differ. 2016 Dec;23(12):1952-1960.

Coupled ATPase-adenylate kinase activity in ABC transporters
Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C, Nat Commun. 2016 Dec 22;7:13864.

Atomic-resolution structure of a disease-relevant Aβ(1-42) amyloid fibril
Wälti MA, Ravotti F, Arai H, Glabe CG, Wall JS, Böckmann A, Güntert P, Meier BH, Riek R., Proc Natl Acad Sci U S A. 2016 Aug 23;113(34).

The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation
von Delbrück M, Kniss A, Rogov VV, Pluska L, Bagola K, Löhr F, Güntert P, Sommer T, Dötsch V., Mol Cell. 2016 Jun 16;62(6):918-28.

Influence of Arrestin on the Photodecay of Bovine Rhodopsin
Chatterjee D, Eckert CE, Slavov C, Saxena K, Fürtig B, Sanders CR, Gurevich VV, Wachtveitl J, Schwalbe H., Angew Chem Int Ed Engl. 2015 Nov 9;54(46):13555-60.

Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition
Luh LM, Hänsel R, Löhr F, Kirchner DK, Krauskopf K, Pitzius S, Schäfer B, Tufar P, Corbeski I, Güntert P, Dötsch V., J Am Chem Soc. 2013 Sep 18;135(37):13796-803.

Peak picking multidimensional NMR spectra with the contour geometry based algorithm CYPICK
Würz JM, Güntert P, J Biomol NMR. 2017 Jan;67(1):63-76.

Atomic-resolution structure of a disease-relevant Aβ(1-42) amyloid fibril
Wälti MA, Ravotti F, Arai H, Glabe CG, Wall JS, Böckmann A, Güntert P, Meier BH, Riek R., Proc Natl Acad Sci U S A. 2016 Aug 23;113(34).

The Exact NOE as an Alternative in Ensemble Structure Determination
Vögeli B, Olsson S, Güntert P, Riek R., Biophys J. 2016 Jan 5;110(1):113-26.

NMR structure calculation for all small molecule ligands and non-standard residues from the PDB Chemical Component Dictionary
Yilmaz EM, Güntert P., J Biomol NMR. 2015 Sep;63(1):21-37.

Combined automated NOE assignment and structure calculation with CYANA
Güntert P, Buchner L, J Biomol NMR. 2015 Aug;62(4):453-71.

Systematic evaluation of combined automated NOE assignment and structure calculation with CYANA
Buchner L, Güntert P, J Biomol NMR. 2015 May;62(1):81-95.

Increased reliability of nuclear magnetic resonance protein structures by consensus structure bundles
Buchner L, Güntert P., Structure. 2015 Feb 3;23(2):425-34.

Automated resonance assignment of the 21kDa stereo-array isotope labeled thioldisulfide oxidoreductase DsbA
Schmidt E, Ikeya T, Takeda M, Löhr F, Buchner L, Ito Y, Kainosho M, Güntert P., J Magn Reson. 2014 Oct 17;249C:88-93.

Cell-free expression of membrane proteins
Cell-free (CF) expression systems have emerged in recent times as promising tools in order to accelerate and to streamline MP expression approaches. The elimination of a living host environment…