Research at the BMRZ

 

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins

Laguerre A, Löhr F, Henrich E, Hoffmann B, Abdul-Manan N, Connolly PJ, Perozo E, Moore JM, Bernhard F, Dötsch V., Structure. 2016 Oct 4;24(10):1830-1841.

Influence of Arrestin on the Photodecay of Bovine Rhodopsin

Chatterjee D, Eckert CE, Slavov C, Saxena K, Fürtig B, Sanders CR, Gurevich VV, Wachtveitl J, Schwalbe H., Angew Chem Int Ed Engl. 2015 Nov 9;54(46):13555-60.

In-cell NMR and EPR spectroscopy of biomacromolecules

Hänsel R, Luh LM, Corbeski I, Trantirek L, Dötsch V., Angew Chem Int Ed Engl. 2014 Sep 22;53(39):10300-14.

Coupled ATPase-adenylate kinase activity in ABC transporters

Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C, Nat Commun. 2016 Dec 22;7:13864.

Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy

Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C, Proc Natl Acad Sci U S A. 2015, 112, 9896-901.

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR

Maciejko J, Mehler M, Kaur J, Lieblein T, Morgner N, Ouari O, Tordo P, Becker-Baldus J, Glaubitz C., J Am Chem Soc. 2015, 137, 9032-43.

The Structural Basis of the Green-Blue Color Switching in Proteorhodopsin determined by NMR Spectroscopy

Mao, Jiafei; Do, Nhu-Nguyen; Scholz, Frank; Reggie, Lenica; Mehler, Michaela; Lakatos, Andrea; Ong, Yean-Sin; Ullrich, Sandra; Brown, Lynda; Brown, Richard; Becker-Baldus, Johanna; Wachtveitl, Josef; Glaubitz, Clemens, J Am Chem Soc. 2014, 136, 17578-90.

Heteronuclear Cross-Relaxation under Solid-State Dynamic Nuclear Polarization

Daube D, Aladin V, Heiliger J, Wittmann JJ, Barthelmes D, Bengs C, Schwalbe H, Corzilius B., J Am Chem Soc. 2016 Dec 28;138(51):16572-16575.

Gd(iii) and Mn(ii) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins

Kaushik M, Bahrenberg T, Can TV, Caporini MA, Silvers R, Heiliger J, Smith AA, Schwalbe H, Griffin RG, Corzilius B., Phys Chem Chem Phys. 2016 Oct 21;18(39):27205-27218.

Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids

Corzilius B., Phys Chem Chem Phys. 2016 Oct 21;18(39):27190-27204.

Dynamic nuclear polarization of nucleic acid with endogenously bound manganese

Wenk P, Kaushik M, Richter D, Vogel M, Suess B, Corzilius B., J Biomol NMR. 2015 Sep;63(1):97-109.

Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy

Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C, Proc Natl Acad Sci U S A. 2015, 112, 9896-901.

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR

Maciejko J, Mehler M, Kaur J, Lieblein T, Morgner N, Ouari O, Tordo P, Becker-Baldus J, Glaubitz C., J Am Chem Soc. 2015, 137, 9032-43.

The Structural Basis of the Green-Blue Color Switching in Proteorhodopsin determined by NMR Spectroscopy

Mao, Jiafei; Do, Nhu-Nguyen; Scholz, Frank; Reggie, Lenica; Mehler, Michaela; Lakatos, Andrea; Ong, Yean-Sin; Ullrich, Sandra; Brown, Lynda; Brown, Richard; Becker-Baldus, Johanna; Wachtveitl, Josef; Glaubitz, Clemens, J Am Chem Soc. 2014, 136, 17578-90.

Dynamic nuclear polarization-enhanced NMR on aligned lipid bilayers at ambient temperature

Jakdetchai O, Denysenkov V, Becker-Baldus J, Dutagaci B, Prisner TF, Glaubitz C, J Am Chem Soc. 2014, 136(44):15533-6.

Dynamic nuclear polarization of (1)H, (13)C, and (59)Co in a tris(ethylenediamine)cobalt(III) crystalline lattice doped with Cr(III)

Corzilius B, Michaelis VK, Penzel SA, Ravera E, Smith AA, Luchinat C, Griffin RG., J Am Chem Soc. 2014 Aug 20;136(33):11716-27.

Detecting substrates bound to the secondary multidrug efflux pump EmrE by DNP-enhanced solid-state NMR

Ong YS, Lakatos A, Becker-Baldus J, Pos KM, Glaubitz C, J Am Chem Soc. 2013, 135, 15754-62.

Mechanism of TAp73 inhibition by ΔNp63 and structural basis of p63/p73 hetero-tetramerization

Gebel J, Luh LM, Coutandin D, Osterburg C, Löhr F, Schäfer B, Frombach AS, Sumyk M, Buchner L, Krojer T, Salah E, Mathea S, Güntert P, Knapp S, Dötsch V.,  Cell Death Differ. 2016 Dec;23(12):1930-1940.

Design of photocaged puromycin for nascent polypeptide release and spatiotemporal monitoring of translation

Buhr F, Kohl-Landgraf J, tom Dieck S, Hanus C, Chatterjee D, Hegelein A, Schuman EM, Wachtveitl J, Schwalbe H., Angew Chem Int Ed Engl. 2015 Mar 16;54(12):3717-21.

Coupled ATPase-adenylate kinase activity in ABC transporters

Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C, Nat Commun. 2016 Dec 22;7:13864.

Selective High-Resolution Detection of Membrane Protein-Ligand Interaction in Native Membranes Using Trityl-Nitroxide PELDOR

Joseph B, Tormyshev VM, Rogozhnikova OY, Akhmetzyanov D, Bagryanskaya EG, Prisner TF., Angew Chem Int Ed Engl. 2016 Sep 12;55(38):11538-42.

Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy

Becker-Baldus J, Bamann C, Saxena K, Gustmann H, Brown LJ, Brown RC, Reiter C, Bamberg E, Wachtveitl J, Schwalbe H, Glaubitz C, Proc Natl Acad Sci U S A. 2015, 112, 9896-901.

Visualizing Specific Cross-Protomer Interactions in the Homo-Oligomeric Membrane Protein Proteorhodopsin by Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR

Maciejko J, Mehler M, Kaur J, Lieblein T, Morgner N, Ouari O, Tordo P, Becker-Baldus J, Glaubitz C., J Am Chem Soc. 2015, 137, 9032-43.

Distance Measurement on an Endogenous Membrane Transporter in E. coli Cells and Native Membranes Using EPR Spectroscopy

Joseph B, Sikora A, Bordignon E, Jeschke G, Cafiso DS, Prisner TF., Angew Chem Int Ed Engl. 2015 May 18;54(21):6196-9.

The Structural Basis of the Green-Blue Color Switching in Proteorhodopsin determined by NMR Spectroscopy

Mao, Jiafei; Do, Nhu-Nguyen; Scholz, Frank; Reggie, Lenica; Mehler, Michaela; Lakatos, Andrea; Ong, Yean-Sin; Ullrich, Sandra; Brown, Lynda; Brown, Richard; Becker-Baldus, Johanna; Wachtveitl, Josef; Glaubitz, Clemens, J Am Chem Soc. 2014, 136, 17578-90.

A Stably Protonated Adenine Nucleotide with a Highly Shifted pKa Value Stabilizes the Tertiary Structure of a GTP-Binding RNA Aptamer

Wolter AC, Weickhmann AK, Nasiri AH, Hantke K, Ohlenschläger O, Wunderlich CH, Kreutz C, Duchardt-Ferner E, Wöhnert J., Angew Chem Int Ed Engl. 2017 Jan 2;56(1):401-404.

An intermolecular G-quadruplex as the basis for GTP recognition in the class V-GTP aptamer

Nasiri AH, Wurm JP, Immer C, Weickhmann AK, Wöhnert J., RNA. 2016 Nov;22(11):1750-1759.

Understanding How DNA Enzymes Work

Wirmer-Bartoschek J, Schwalbe H., Angew Chem Int Ed Engl. 2016 Apr 25;55(18):5376-7.

What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch

Duchardt-Ferner E, Gottstein-Schmidtke SR, Weigand JE, Ohlenschläger O, Wurm JP, Hammann C, Suess B, Wöhnert J., Angew Chem Int Ed Engl. 2016 Jan 22;55(4):1527-30.

Involvement of Long-Lived Intermediate States in the Complex Folding Pathway of the Human Telomeric G-Quadruple

Bessi I, Jonker HR, Richter C, Schwalbe H., Angew Chem Int Ed Engl. 2015 Jul 13;54(29):8444-8.

Building a stable RNA U-turn with a protonated cytidine

Gottstein-Schmidtke SR, Duchardt-Ferner E, Groher F, Weigand JE, Gottstein D, Suess B, Wöhnert J., RNA. 2014 Aug;20(8):1163-72.

Essential ribosome assembly factor Fap7 regulates a hierarchy of RNA-protein interactions during small ribosomal subunit biogenesis

Hellmich UA, Weis BL, Lioutikov A, Wurm JP, Kaiser M, Christ NA, Hantke K, Kötter P, Entian KD, Schleiff E, Wöhnert J., Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15253-8.

Three-state mechanism couples ligand and temperature sensing in riboswitches

Reining A, Nozinovic S, Schlepckow K, Buhr F, Fürtig B, Schwalbe H., Nature. 2013 Jul 18;499(7458):355-9.

Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family

Kehrloesser S, Osterburg C, Tuppi M, Schäfer B, Vousden KH, Dötsch V., Cell Death Differ. 2016 Dec;23(12):1952-1960.

Coupled ATPase-adenylate kinase activity in ABC transporters

Kaur H, Lakatos-Karoly A, Vogel R, Nöll A, Tampé R, Glaubitz C, Nat Commun. 2016 Dec 22;7:13864.

The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation

von Delbrück M, Kniss A, Rogov VV, Pluska L, Bagola K, Löhr F, Güntert P, Sommer T, Dötsch V., Mol Cell. 2016 Jun 16;62(6):918-28.

Influence of Arrestin on the Photodecay of Bovine Rhodopsin

Chatterjee D, Eckert CE, Slavov C, Saxena K, Fürtig B, Sanders CR, Gurevich VV, Wachtveitl J, Schwalbe H., Angew Chem Int Ed Engl. 2015 Nov 9;54(46):13555-60.

Molecular crowding drives active Pin1 into nonspecific complexes with endogenous proteins prior to substrate recognition

Luh LM, Hänsel R, Löhr F, Kirchner DK, Krauskopf K, Pitzius S, Schäfer B, Tufar P, Corbeski I, Güntert P, Dötsch V., J Am Chem Soc. 2013 Sep 18;135(37):13796-803.

Cell-free expression of membrane proteins

Cell-free (CF) expression systems have emerged in recent times as promising tools in order to accelerate and to streamline MP expression approaches. The elimination of a living host environment…