Development of a lanthanide binding tag for NMR und X-ray based protein structure determination

We developed together with B. Imperiali (MIT) a universal lanthanide binding tag that can improve NMR protein structures by the measurement of residual dipolar couplings (RDCs) and X-ray structures by heavy metal derivatives.

  • Silvaggi, N. R., Martin, L. J., Schwalbe, H., Imperiali, B. & Allen, K. N. Double-lanthanide-binding tags for macromolecular crystallographic structure determination. J. Am. Chem. Soc. 129, 7114-7120 (2007)
  • Martin, L. J., Hähnke, M. J., Nitz, M., Wöhnert, J., Silvaggi, N. R., Allen, K. N., Schwalbe, H. & Imperiali, B. Double-lanthanide-binding tags: Design, photophysical properties, and NMR applications. J. Am. Chem. Soc. 129, 7106-7113 (2007)